Journal of Pure and Applied MicrobiologyVol. 8 No. 6

Isolation, Identification, Optimal Culture Conditions, and Enzyme Production of a Wood-decay Fungus Flavodon sp. GSM-11

Xiao-Meng Zhao1, Yong Bian2, Lun Yan1, Qing-Jun Chen1 and Guo-Qing Zhang1*

1 Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Sciences and Engineering, Beijing University of Agriculture, Beijing - 102206, China. 2 Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing - 100026, China.

Received on 03 August 2014 and accepted on 06 September 2014

 

ABSTRACT

A wood-decay fungus Flavodon sp. GSM-11 was isolated from imported wood Gluta tavoyana of Burma. It was identified as F. flavus based on ITS gene amplification and phylogenetic analysis. Culture characteristics of mycelia showed that it demonstrated favorite carbon source of glucose, favorite nitrogen sources of soybean meal and silkworm pupa meal, favorite C/N ratio of 10/1, optimal temperature of 36 ?C, and optimal pH of 7.0, respectively. Undergoing an 8 day liquid fermentation with PD medium, F. flavus manifested considerably high extracellular laccase activity both in mycelia and in broth. Copper ions can stimulate laccase production at a concentration range of 1.0-5.0 mmol/L. The highest laccase production (485.0 ? 19.2 U/mL) was obtained with a Cu2+ adjunction concentration of 5.0 mM at 6th day of the fermentation. The strain also produced phytase, protease, and RNase with low levels, but was devoid of lectin.

Keywords : Culture condition, Enzyme production, Flavodon flavus, ITS identification, Laccase.